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Lung-Targeted Transgene Expression of Nanocomplexed Ad5 Enhances Immune Response in the Presence of Preexisting Article

Yilong Yang, Shipo Wu, Yudong Wang, Fangze Shao, Peng Lv, Ruihua Li, Xiaofan Zhao, Jun Zhang, Xiaopeng Zhang, Jianmin Li, Lihua Hou, Junjie Xu, Wei Chen

Engineering 2023, Volume 27, Issue 8,   Pages 127-139 doi: 10.1016/j.eng.2022.12.007

Abstract: nanoparticles ((+)HSAnp), which are capable of forming a complex with Ad5, significantly increase the transgeneexpression of Ad5 in both coxsackievirus-adenovirus receptor-positive and -negative cells.manners, Ad5/(+)HSAnp complexes achieve robust (up to 227-fold higher) and long-term (up to 60 days) transgeneexpression in the lungs of mice following intranasal instillation.

Keywords： Adenovirus serotype 5     Vaccine     Preexisting immunity     Nanoparticles     Transgene expression    

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Blockage of receptor-interacting protein 2 expression by small interfering RNA in murine macrophages

LIU Hongchun, CAO Zhongwei, JIN Jianjun, WANG Jiyao

Frontiers of Medicine 2008, Volume 2, Issue 2,   Pages 166-170 doi: 10.1007/s11684-008-0030-1

Abstract: This study aims to demonstrate that blocking the receptor-interacting protein2 (Rip2) expression canRip2 small interfering RNA (siRNA) plasmids were constructed and transfected into macrophage and Rip2 expressionRip2 siRNA plasmid could block the mRNA and protein expression of Rip2 and promote cell proliferationThe HMGB1 expression in the liver decreased to (40.21 ± 11.03) pg/g, and serum TNF-?The results demonstrate that Rip2 siRNA plasmid can block the expression of Rip2, decrease the production
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MSI/LOH and extron expression of the FHIT gene in gastric carcinoma

XIAO Yuping, MAO Lili, HAN Chengbo, LI Jinyi, XU Lei, XIN Yan

Frontiers of Medicine 2007, Volume 1, Issue 1,   Pages 99-103 doi: 10.1007/s11684-007-0019-1

Abstract: We detected loss of heterozygosity (LOH) and microsatellite instabilities (MSI), as well as extron expressionFour of 10 (40.0%) cases of primary GC showed absent or decreased expression of the FHIT protein as comparedThe findings in this study suggest that LOH and MSI of FHIT gene may induce aberrant extron expression
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Gene expression disparity in giant cell tumor of bone

Xiaohua PAN, Shuhua YANG, Deming XIAO, Yong DAI, Lili REN

Frontiers of Medicine 2009, Volume 3, Issue 1,   Pages 49-56 doi: 10.1007/s11684-009-0012-y

Abstract: The aim of this paper was to study the differential gene expression of giant cell tumor of bone (GCTBThe gene expression disparity between the GCTB samples and normal bony callus was significantly different

Keywords： giant cell tumor of bone     gene     microarray     cDNA    

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Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation

Frontiers of Medicine 2022, Volume 16, Issue 4,   Pages 627-636 doi: 10.1007/s11684-020-0815-4

Abstract: Here, based on 1741 patients with AML, we report a unique expression pattern associated with RUNX1This expression pattern was coordinated by target repression and promoter hypermethylation.This study revealed the distinct gene expression pattern of RUNX1 mutations and the underlying

Keywords： RUNX1     gene mutation     acute myeloid leukemia     transcriptional repression     DNA methylation    

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Immunohistochemical measurement and expression of Mcl-1 in infertile testes

Raafat Hegazy,Abdelmonem Hegazy,Mustafa Ammar,Emad Salem

Frontiers of Medicine 2015, Volume 9, Issue 3,   Pages 361-367 doi: 10.1007/s11684-015-0395-x

Abstract: This study aimed to investigate the immunohistochemical expression of Mcl-1 in testicular biopsies of

Keywords： spermatogenesis     testis     Mcl-1 expression     immunohistochemical study     infertility    

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Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 382-389 doi: 10.15302/J-FASE-2018211

Abstract: A key component in the technology is the construction of an efficient milk expression vector.Here, we established a simple method to construct a milk expression vector, by a combination of homologousThe highest expression level of rhLZ was 0.45 g·L−1, and rhLZ exhibited the sameOur results have provided a simple approach to construct a universal milk expression vector, and demonstratedthat the resulting vector regulates the expression of hLZ in milk.

Keywords： BAC recombinant methods     gene expression     human lysozyme     transgenic mice     milk expression vector    

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Relationship of adrenomedullin expression and microvessel density and prognosis in smooth muscle tumor

JIANG Yuan, TIAN Xuehong, YUAN Jie, JIN Yuemei, TAN Yusong

Frontiers of Medicine 2007, Volume 1, Issue 4,   Pages 398-400 doi: 10.1007/s11684-007-0077-4

Abstract: The aim of this paper was to investigate the relationship between the expression of adrenomedullin (ADMThe expression of ADM was detected using immunohistochemical staining in specimens from 15 normal controlsThere was a positive correlation between the ADM expression and MVD in LE and LES respectively ( = 0.823The expression of ADM in LE was statistically lower than that in LES (<0.05).mitotic figures, the higher levels of the ADM expression and poor prognosis.

Keywords： anticarcinogenic     microvessel density     malignant potency     muscle     uterine leiomyosarcoma    

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Heterologous expression of signal protein 14-3-3 in and the subsequent immune response in mice

ZHENG Meijuan, SHEN Jilong, LUO Qingli, XU Yuanhong

Frontiers of Medicine 2008, Volume 2, Issue 1,   Pages 95-99 doi: 10.1007/s11684-008-0017-y

Abstract: The Sj14-3-3 gene was amplified and subcloned into the expression vector pPICZ?The recombinant Sj14-3-3 of eukaryotic expression in was successfully harvested.

Keywords： development     challenging     rSj14-3-3     resistance     cultural supernatant    

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Plant genetic engineering and genetically modified crop breeding: history and current status

Xingchun WANG,Shujun CHANG,Jie LU,Rupert FRAY,Don GRIERSON,Yuanhuai HAN

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 1,   Pages 5-27 doi: 10.15302/J-FASE-2017131

Abstract: This review charts the major developments in the genetic manipulation of plant cells that have taken place since the first gene transfer experiments using Ti plasmids in 1983. Tremendous progress has been made in both our scientific understanding and technological capabilities since the first genetically modified (GM) crops were developed with single gene resistances to herbicides, insects, viruses, and the silencing of unde-sirable genes. Despite opposition in some parts of the world, the area planted with first generation GM crops has grown from 1.7 Mhm in 1996 to 179.7 Mhm hectares in 2015. The toolkit available for genetic modification has expanded greatly since 1996 and recently Nobel Laureates have called on Greenpeace to end their blanket opposition, and plant scientists have urged that consideration be given to the benefits of GM crops based on actual evidence. It is now possible to use GM to breed new crop cultivars resistant to a much wider range of pests and diseases, and to produce crops better able to adapt to climate change. The advent of new CRISPR-based technologies makes it possible to contemplate a much wider range of improvements based on transfer of new metabolic pathways and traits to improve nutritional quality, with a much greater degree of precision. Use of GM, sometimes in conjunction with other approaches, offers great opportunities for improving food quality, safety, and security in a changing world.

Keywords： plant transformation     transgene     genetic modification    

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Increased expression of coronin-1a in amyotrophic lateral sclerosis: a potential diagnostic biomarker

Frontiers of Medicine 2022, Volume 16, Issue 5,   Pages 723-735 doi: 10.1007/s11684-021-0905-y

Abstract: Among them, the expression of exosomal coronin-1a (CORO1A) was 5.3-fold higher than that in the controlsoverexpressed in NSC-34 motor neuron-like cells, and apoptosis, oxidative stress, and autophagic protein expression

Keywords： amyotrophic lateral sclerosis     coronin-1a     autophagy     pathogenesis    

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Effect of arsenic trioxide on proliferation and apoptosis of U266 cells and its relationship with the expression

ZHAN Rong, YU Qinghong, HUANG Haobo

Frontiers of Medicine 2008, Volume 2, Issue 4,   Pages 356-360 doi: 10.1007/s11684-008-0068-0

Abstract: trioxide (AsO) on the proliferation and apoptosis of myeloma cell line U266 and its relationship with the expressionThe effect of AsO on the VEGF expression of U266 cells were tested by enzyme linked immunosorbent assayAs far as single cells are concerned, nevertheless, the expression of VEGF mRNA did not vary.decrease the tumor load, and lead to the reduction of VEGF in the culture supernatant, but not change the expression

Keywords： VEGF expression     expression variation     culture supernatant     labeling     concentration    

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Relationship between expression of hepatocyte grow factor and apoptosis of trophoblasts in hypertensive

OUYANG Shan, ZHANG Qinghua, QIAO Fuyuan

Frontiers of Medicine 2007, Volume 1, Issue 4,   Pages 386-389 doi: 10.1007/s11684-007-0075-6

Abstract: The aim of this study was to investigate the expression of hepatocyte growth factor (HGF) and Fas inThe expression of HGF mRNA in mild preeclampsia, severe preeclampsia and eclampsia cases was significantlythat in the uncomplicated cases (0.43±0.12, 0.38±0.09, 0.19±0.17 versus 0.67±0.19, <0.05), while the expressionThese results indicate that HGF inhibits the apoptosis mediated by Fas, and the reduced expression of

Keywords： pregnancy     concentration     possible relationship     responsible     hepatocyte    

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Relative expression of PTTG and bFGF in oral squamous cell carcinoma and Tca8113

Yumei DING BM , Lili CHEN MD , Bo CHENG PhD , Handong ZHANG MM ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 357-362 doi: 10.1007/s11684-009-0046-1

Abstract: The purpose of this study was to investigate the expression of pituitary tumor transforming gene (PTTGadditionally, after being treated with different concentrations of anti bFGF or PTTG antibody, PTTG or bFGF expressionPTTG protein expression was positively correlated with bFGF ( = 0.382, <0.05); (2) PTTG protein emittedAnti-PTTG antibody also had an inhibitive effect on bFGF expression.PTTG protein expression conforms to bFGF in OSCC tissues and Tca8113 cells.

Keywords： carcinoma     squamous cell     pituitary tumor transforming gene (PTTG) protein     basic fibroblast growth factor    

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Expression and bioinformatic analysis of lymphoma-associated novel gene KIAA0372

BAI Xiangyang, TANG Duozhuang, ZHU Tao, SUN Lishi, YAN Lingling, LU Yunping, ZHOU Jianfeng, MA Ding

Frontiers of Medicine 2007, Volume 1, Issue 1,   Pages 93-98 doi: 10.1007/s11684-007-0018-2

Abstract: lymphomas and reactive lymph node hyperplasia were respectively marked with biotin and hybridized with Gene ExpressionUsing Gene Expression Chips, many differentially expressed genes were uncovered.

Keywords： bioinformatic analysis     functional     KIAA0372     detection     Microarray    

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Title Author Date Type Operation

Lung-Targeted Transgene Expression of Nanocomplexed Ad5 Enhances Immune Response in the Presence of Preexisting

Yilong Yang, Shipo Wu, Yudong Wang, Fangze Shao, Peng Lv, Ruihua Li, Xiaofan Zhao, Jun Zhang, Xiaopeng Zhang, Jianmin Li, Lihua Hou, Junjie Xu, Wei Chen

Journal Article

Blockage of receptor-interacting protein 2 expression by small interfering RNA in murine macrophages

LIU Hongchun, CAO Zhongwei, JIN Jianjun, WANG Jiyao

Journal Article

MSI/LOH and extron expression of the FHIT gene in gastric carcinoma

XIAO Yuping, MAO Lili, HAN Chengbo, LI Jinyi, XU Lei, XIN Yan

Journal Article

Gene expression disparity in giant cell tumor of bone

Xiaohua PAN, Shuhua YANG, Deming XIAO, Yong DAI, Lili REN

Journal Article

Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation

Journal Article

Immunohistochemical measurement and expression of Mcl-1 in infertile testes

Raafat Hegazy,Abdelmonem Hegazy,Mustafa Ammar,Emad Salem

Journal Article

Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Journal Article

Relationship of adrenomedullin expression and microvessel density and prognosis in smooth muscle tumor

JIANG Yuan, TIAN Xuehong, YUAN Jie, JIN Yuemei, TAN Yusong

Journal Article

Heterologous expression of signal protein 14-3-3 in and the subsequent immune response in mice

ZHENG Meijuan, SHEN Jilong, LUO Qingli, XU Yuanhong

Journal Article

Plant genetic engineering and genetically modified crop breeding: history and current status

Xingchun WANG,Shujun CHANG,Jie LU,Rupert FRAY,Don GRIERSON,Yuanhuai HAN

Journal Article

Increased expression of coronin-1a in amyotrophic lateral sclerosis: a potential diagnostic biomarker

Journal Article

Effect of arsenic trioxide on proliferation and apoptosis of U266 cells and its relationship with the expression

ZHAN Rong, YU Qinghong, HUANG Haobo

Journal Article

Relationship between expression of hepatocyte grow factor and apoptosis of trophoblasts in hypertensive

OUYANG Shan, ZHANG Qinghua, QIAO Fuyuan

Journal Article

Relative expression of PTTG and bFGF in oral squamous cell carcinoma and Tca8113

Yumei DING BM , Lili CHEN MD , Bo CHENG PhD , Handong ZHANG MM ,

Journal Article

Expression and bioinformatic analysis of lymphoma-associated novel gene KIAA0372

BAI Xiangyang, TANG Duozhuang, ZHU Tao, SUN Lishi, YAN Lingling, LU Yunping, ZHOU Jianfeng, MA Ding

Journal Article